Two weeks after Dox removal, at 5 weeks of age, quantitative real-time PCR (qPCR) analyses indicated that Wls expression was significantly decreased in tumor-bearing bones from WlsΔOB-OS mice compared to Wls floxed, H2k-c-fosLTR littermates that do not carry the Osx-tetO-cre allele (WlsWT-OS), consistent with activation of Cre expression (Fig. 1b). This evidence concerns the gene SP7 and neoplasm.