The high abundance of major immune cell subtypes in NPC was then confirmed by multiplex immunofluorescence analysis (Supplementary information, Fig. S3), especially for the T/NK cells, and the mean percentages of CD4+ T, CD8+ T, and CD57+ NK cells in all cells were 19%, 15% and 2%, respectively. The gene discussed is CD8A; the disease is nasopharyngeal carcinoma.