Although DC1, FCRL4+ B, GC B and plasma cells were significantly associated with a relatively high number of mutations, most immune cell subtypes generally correlated poorly with mutational load (Fig. 7c), probably due to the overall low rate of non-synonymous mutations in NPC.7,9 With respect to tumor staging, a general reduction in many immune signatures was shown in patients with relatively high T and N categories and clinical stages (Supplementary information, Fig. S6b). The gene discussed is ZDHHC4; the disease is neoplasm.