The DNA methylation levels for DCAF4L2, CKLF and UBE2C in T samples were not significantly different from those in viral hepatitis-related HCCs, whereas the DNA methylation levels for TRIM4, PRC1 and TUBA1B in T samples were significantly lower than those in viral hepatitis-related HCCs, indicating that DNA hypomethylation of the TRIM4, PRC1 and TUBA1B genes occurred specifically during NASH-related hepatocarcinogenesis (Fig. 3). The gene discussed is TUBA1B; the disease is animal viral hepatitis.