Using the orthotopic osteosarcoma metastasis model in vivo, lung metastases of U2OS/MTX300-Luc cells stably expressing Rab22a-NeoF1, but not Rab22a-NeoF1-K7R, were dramatically enhanced compared to those stably expressing Vector (Figure 2C-G). This evidence concerns the gene RAB22A and osteosarcoma.