ANXA5 and osteosarcoma: Like F-AgÅPs, DCA caused significant increases in ROS production, decreases in cell viability and ΔΨm, as well as increases in osteosarcoma cell apoptosis, as indicated by DCFH-DA staining assay (Figure 8A), CCK-8 assay (Figure 8B), live/dead cell staining (Figure 8, C and D), JC-1 staining (Figure 8E) and annexin V-FITC/PI staining with flow cytometry (Figure 8F), respectively.