To test the idea that exosomes may be critical for GLP-2 induced proliferative and anti-apoptotic effects in short bowel syndrome, we isolated and purified intestinal exosomes from jejunum of saline treated SBS rats, GLP-2 treated SBS rats and sham-operated rats (called SBS-Exo, GLP2-Exo and Sham-Exo respectively) (Figure 2A). This evidence concerns the gene GCG and short bowel syndrome.