We found MYH9 was mainly located in the GC cell nuclei, and presented the first evidence showing nuclear MYH9, with special nuclear localization signals and a DNA-binding domain, directly bound to the CTNNB1 promoter to induce β-catenin transcription and increase activation of the canonical Wnt/β-catenin signaling pathway, which equipped GC cells with anoikis resistance and promoted GC metastasis. The gene discussed is MYH9; the disease is gastric cancer.