With qRT-PCR assay, we found that there was no significant alteration of the level of MYC mRNA in Hep3B, HepG2 and SMMC7721 HCC cells with overexpression of lncCSMD1 compared with the corresponding control cells (Figure 5A left), indicating that lncCSMD1 does not regulate MYC at the transcriptional level. This evidence concerns the gene MYC and hepatocellular carcinoma.