Flow cytometry analysis indicated that knocking down MCM3AP-AS1 promoted cell apoptosis compared with the sh-NC group (Fig. 2f). Consistently, compared with the control group, Bax expression in MCM3AP-AS1 knockdown group increased markedly, while the expression of Bcl-2 decreased (Fig. 2g), suggesting that MCM3AP-AS1 was involved in the apoptosis of PCa cells. Here, BAX is linked to posterior cortical atrophy.