Moderate–strong correlations were found for both CD3 and CD8 IHC quantified in the invasive margin and central tumour (CD3 invasive margin R2 = 0.65, P < 0.0001; CD3 central tumour R2 = 0.94, P < 0.0001; CD8 invasive margin R2 = 0.66, P < 0.0001; CD8 central tumour R2 = 0.97, P < 0.0001; Supplementary Fig. S2B–E). The gene discussed is CD8A; the disease is neoplasm.