TLR8 and obesity due to melanocortin 4 receptor deficiency: We applied the Attie Lab Diabetes database to verify the hub gene mRNA levels in obesity, which indicated that expression of TYROBP, TLR8, FCER1 G, HCK, NCF2, CTSS and C3AR1 was significantly up-regulated in 10-week obese mice as compared to the lean group (Figure 9).