Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that deletion of mutant ASXL1 allele significantly (P < 0.01) up-regulated genes enriched in JAK-STAT signaling pathway, Cytokine-cytokine receptor interaction, FoxO signaling pathway, NF-kappa B signaling pathway, and transcriptional misregulation in cancer, while significantly (P < 0.05) down-regulated genes were enriched in hematopoietic cell lineage, regulation of actin cytoskeleton, Fc gamma R-mediated phagocytosis, Hippo signaling pathway and alcoholism (Fig. 3A and Table S3). This evidence concerns the gene ASXL1 and alcohol dependence.