Both OD 450 values and the proportion of Ki67-positive cells in DM1-03+Ad-MBNL1+rapamycin SSCs were significantly lower than those in DM1-03+Ad-MBNL1 SSCs (Fig. 6c, g, h), whereas there were no significant differences between DM1-03+Ad-MBNL1+rapamycin and DM1-03 SSCs (Fig. 6c, g, h). Here, MKI67 is linked to myotonic dystrophy type 1.