To investigate whether MBNL1 promotes proliferation by inhibiting autophagy via the mTOR pathway in DM1 SSCs, we performed western blot analysis, CCK-8 assays, and Ki67 immunofluorescence to examine the proliferation of DM1-03+Ad-MBNL1 and DM1-13-3+Ad-MBNL1 SSCs after rapamycin treatment. The gene discussed is MBNL1; the disease is myotonic dystrophy type 1.