IPP and neoplasm: To investigate whether ascites-induced IPP influences early events in adhesion of OvCa cells to peritoneum, an in vivo artificial ascites assay was developed in which C57Bl/6 female mice were injected i.p. with RFP-tagged OVCAR5 or OVCAR8 cells in a large volume (5 mL) of PBS to mimic the ascites condition, relative to control mice that received tumor cells in a small volume (1 mL) of PBS (Fig. 1a)20.