To determine if hepatic TFEB activation induces bile acid synthesis and improves cholesterol homeostasis, we subjected TFEB overexpressing mice and controls to WD feeding for 1 week, a feeding length that markedly increased hepatic and plasma cholesterol (Fig. 8a, b) but was not long enough to cause appreciable hepatic TG accumulation or hypertriglyceridemia (Supplementary Fig. 9a, b), allowing us to study cholesterol changes independent of TFEB-mediated reduction of hepatic steatosis26. This evidence concerns the gene TFEB and hypertriglyceridemia.