In our study, we did not detect any obvious changes in the autophagic proteins Beclin1 and Atg-5, but when we used the mRFP-GFP-LC3 adenovirus as a marker, we found that itraconazole could promote the autophagic flux of colon cancer cells, indicating that this effect might has been caused by a reduction in shh activity. The gene discussed is ATG5; the disease is colonic neoplasm.