CYP11A1 and neoplasm: To reveal the gene expression identity of intratumoral steroidogenic immune cells and patterns of gene expression at single-cell resolution, we inoculated B16-F10 subcutaneous tumor in Cyp11a1-mCherry reporter mice, enriched and purified intratumoral Cyp11a1-mCherry+ and Cyp11a1-mCherry− cells by cell sorting into 96-well plates [with a ratio of 79:15 (mCherry+: mCherry−) cells per plate] and performed single-cell RNA sequencing (scRNA-seq) using the SMART-Seq2 platform40.