SMAD4 and pancreatic neoplasm: Pancreatic cancer cell line-conditioned media caused different alterations in the intracellular calcium of PBMCs depending on the presence or complete absence of SMAD4 gene: in the presence (not the absence) of SMAD4, the PDAC conditioned media induced an increase in the number of calcium peaks and a reduction in the whole intracellular calcium (Table 2).