INPP5D and carcinoma: We then performed a SHIP1 phosphatase assay based on the malachite-green dependent detection of phosphate liberated in the course of the SHIP1 catalyzed dephosphorylation of PtdIns(3,4,5)P3 using four SHIP1 expressing model carcinoma cell lines (HT-29, SW-480, SKCHA-1 and HepG2).