Evaluating the role of E7, p65 and Pirin in increased cell migration, a transwell assay was carried out in SCC143/E7 and squamous cell carcinoma of the oral lateral tongue (SCC47) (HPV16-positive cell line) cells previously transfected with a siRNA for E7, p65 or PIR knockdown, with the functionality of corresponding siRNA shown in Figures S1E and S1F. The gene discussed is PIR; the disease is squamous cell carcinoma.