As ESCC tissues hardly expresses AR (Figure 7D), thus, it is reasonable to infer that the metabolites 3α‐diol may be reused to biosynthesis PI, known as a precursor of PI3K,52 following by activating the PI3K/AKT pathway in an AR‐independent manner,43, 44, 45, 46 However, there is insufficient evidence that whether 3α‐diol could biosynthesis PI directory in vivo. This evidence concerns the gene AKT1 and esophageal squamous cell carcinoma.