Moreover, low dosage (1 μg) of mZac1.HA have more significant impact on EMT markers comparing with high dosage (3 μg) in SiHa and Caski (Fig. 7B), suggesting that EMT may optimally induced by moderate level of Zac1 expression, which is corresponds to previous observation comparing normal and cervical cancer with high Zac1 expression (Fig. 4A). The gene discussed is PLAGL1; the disease is cervical carcinoma.