Zac1 expression from 21 cervical cancer tumor were divided into two groups: 50% from top as high Zac1 expression (10 patients) and 50% from bottom as low Zac1 expression (11 patients), in which the mean Zac1 expression in cervical cancer with high Zac1 is similar to normal cervix epithelium, implying that high Zac1 expression may not resulted by amplified copy number in the cancer tissue (Fig. 4A). This evidence concerns the gene PLAGL1 and cancer.