The expression levels of GSK3β and its Y216 phosphorylated fraction (pGSK3βY216, active form) were higher in all ESCC cell lines compared to normal esophageal squamous TYNEK-3 cells (Fig. 1A), with less detectable S9 phosphorylation (pGSK3βS9, inactive form). The gene discussed is GSK3B; the disease is esophageal squamous cell carcinoma.