However, although Maharjan et al. [98] reported that SGs were induced in a majority of unstressed mouse Neuro2A (N2A) neuroblastoma cells when transfected with myc-tagged C9-L, we failed to observe this phenomenon for tagged C9-L or SMCR8 proteins transfected alone or in combination (not shown) in unstressed human osterosarcoma U2OS, HEK 293T, or neuroblastoma cell lines (Fig. 4b, S4B). This evidence concerns the gene SMCR8 and neuroblastoma.