In our validation cohort, we performed IHC staining and we found patients in high FTL expression group had higher level of β-catenin when compared with patients in lower FTL expression group (Figure S4B-C).What’s more, in glioma tissues with high FTL expression, the accumulation of β-catenin in the nucleus was more common than in low FTL expression glioma tissues. The gene discussed is FTL; the disease is central nervous system cancer.