Because TLR1, 2, 4, 5, and 6 are well‐known host receptors recognizing bacterial ligands, TLR 3 is also reported to be involved in skin sensitization53 as well as metal‐induced skin and mucosa irritation reactivity,54, 55 and all these TLRs may play a role in allergic reactions, for example, in asthma,56 we next determined the mRNA expression of TLR1‐6 in order to gain more mechanistic understanding of the differences observed between RHS and RHG upon S. mitis and metal exposure. This evidence concerns the gene TLR1 and asthma.