These markers included mRNA expression of CD3, CD8, GZMB, and IL2. In addition, we focused the analysis of CD3+-based markers in the context of tumor expression of shared antigens belonging to the cancer-testis (CT) antigen family, because their expression is normally restricted to germ cells but they become aberrantly expressed in several tumors types, including ovarian cancer [28]. Here, GZMB is linked to neoplasm.