Meanwhile, a total of 14 KEGG pathways were over-represented for LS, which consisted of several cardiovascular-related pathways such as Hypertrophic cardiomyopathy (KEGG id: hsa05410) and some cancer-related pathways such as PI3K-Akt signaling pathway (KEGG id: hsa04151) (Figure 2B and Supplementary Table S2). This evidence concerns the gene AKT1 and Leigh syndrome.