Given the intratumoral heterogeneity of the primary lesion, we analyzed two different portions of fresh-frozen TISS-P1.M (SETD2mut tissues A and B) and compared the results to those obtained in poorly differentiated thyroid cancer (PDTC) harboring wild-type SETD2. As shown in Figure 2A, the SETD2-wt control tissue displayed a 270-KDa band corresponding to the full-length SETD2 protein and detectable levels of H3K36me3. Here, SETD2 is linked to thyroid cancer.