In subgroups analysis, levels of ESAT6‐specific perforin were significantly increased in PBMCs of LTBI individuals (1.26 ± 0.71 ng/mL) compared to ATB patients (0.67 ± 0.39 ng/mL), healthy controls (0.25 ± 0.36 ng/mL), or non‐TB control subjects (0.09 ± 0.47 ng/mL), (P < .001), (Figure 2B). Here, PRF1 is linked to tuberculosis.