PRF1 and tuberculosis: After stimulation with ESAT6 or CFP10, levels of total perforin were significantly increased in PBMCs of TB group (ESAT6: 2.98 ± 1.29 ng/mL, CFP10: 3.80 ± 1.21 ng/mL) compared with control group (ESAT6: 1.63 ± 0.89 ng/mL, CFP 10:1.75 ± 0.89 ng/mL), (P < .001), (Figure 1A).