PRF1 and tuberculosis: In subgroups analysis, the levels of total perforin were significantly increased in PBMCs of ATB patients (2.79 ± 1.20 ng/mL) and LTBI patients (3.22 ± 1.40 ng/mL) compared to healthy volunteer subjects (1.60 ± 0.71 ng/mL) and non‐TB patients (1.66 ± 1.06 ng/mL) after stimulation with ESAT6 (Figure 1B).