According to the methodology conducted by Wang Feng et al,33 they calculated Mtb‐specific TNF‐α by subtracting the background level of TNF‐α secreted by the unstimulated PBMCs, we found that diagnostic performance of Mtb‐specific perforin was substantially improved compared to total perforin for the diagnosis of TB disease and also discriminates between ATB and LTBI in this study. The gene discussed is PRF1; the disease is tuberculosis.