Target NGS approach was designed to cover entire globin genes coding regions, their key regulatory regions, and modifier genes such as KLF1, BCL11A, HBS1L, and MYB. Preliminary data show that NGS may be much more accurate than convention thalassemia diagnosis by complete blood count (CBC), Hb analysis, Hb typing, and selected for genotyping. Here, KLF1 is linked to thalassemia.