Western blot analysis indicated that the phosphorylation levels of NF-κB p65 and STAT3 were increased in regorafenib-resistant HCC cells (Figure 8A) and SphK2-overexpressing HCC cells (Figure 8D), whereas they were decreased in regorafenib-resistant HCC cells following SphK2 knockdown or inhibition (Figures 8B,C). The gene discussed is SPHK2; the disease is hepatocellular carcinoma.