To prove the effects of TDRKH-AS1 on CRC cell proliferation and tumorigenicity, we designed five different specific small interfering RNAs (siRNAs), siTDRKH-AS1 1 (siT-1) to siT-5, which were able to knock down the expression of the TDRKH-AS1 through silencing it. This evidence concerns the gene TDRKH and colorectal carcinoma.