To further explore the functionalities and evolutionary track of DPT cells, we sorted and performed single‐cell RNA‐seq and TCR‐seq on 5018 tumor‐associated DPT cells (TDPT), 3676 CD4 single positive T cells (TCD4T), 4470 CD8 single positive T cells (TCD8T), and 4652 PBMC CD3+T cells (low‐quality cells were filtered before analysis) (Figure 5A and Figure S5A, Supporting Information). Here, CD4 is linked to neoplasm.