CXCR4 and HIV-1 infection: In addition, as the CXCR4 was another co-receptor of HIV-1 infection, we have also screened and edited CXCR4 in genome successfully with CRISPR/AsCpf1 in TZM.bl cells, and the co-receptors CCR5 and CXCR4 have been ablated simultaneously by co-transfection the corresponding plasmids (AsCpf1-CCR5-#4 and AsCpf1-CXCR4-#2) into TZM.bl cells.