In this study, we screened and identified two CRISPR/AsCpf1 using sgRNAs with high specificity to target CCR5. With different CD4+T cells (Tzm.bl, SupT1-R5, primary CD4+T cells), we demonstrated that CRISPR/AsCpf1 could ablate CCR5 gene efficiently in all cell types and confer the edited cells resistant to R5-tropic strain but not X4-tropic strain infection. The gene discussed is CD4; the disease is infection.