The CRISPR/AsCpf1-#4/#5 have been clarified that they could work efficiently to disrupt CCR5 gene in different cell types of HIV-1 study (TZM.bl, SupT1-R5, primary CD4+T cells).The edited cells, compared with unedited control group showed that they could resist R5-tropic HIV-1 but not X4-tropic HIV-1 infection in all cell types. The gene discussed is CCR5; the disease is HIV-1 infection.