To test putative HF effectors for plant immune suppression, we employed an assay that uses Burkholderia glumae bacteria, and the effector detector vector (pEDV) system to deliver HF candidate Avr proteins to Nicotiana tabacum and N. benthamiana via the bacterial type III secretion system (T3SS) (Sohn et al., 2007; Fabro et al., 2011). This evidence concerns the gene NLRP6 and hydrops fetalis.