In a recent single-cell RNA sequencing (scRNA-seq) study, two distinct subpopulations of liver macrophages are exhibited in western diet (WD)-induced NASH models in mice, including MoMFs with high lysozyme 2 (Lyz2) expression and KCs with high C-type lectin domain family 4 member F (Clec4f) expression (21). This evidence concerns the gene CLEC4F and metabolic dysfunction-associated steatohepatitis.