The known phenotypic effects of TM5441 in vivo include protection against hypertension and vascular senescence in Nω-nitro-l-arginine methyl ester (L-NAME)-induced hypertensive mice (Boe et al., 2013), prolongation of lifespan in klotho null mice (Eren et al., 2014), and improved renal function and morphology in streptozotocin-induced diabetic mice (Jeong et al., 2016). Here, KL is linked to hypertensive disorder.