In addition, qRT-PCR and Western blot assay verified that the expression of LRP6 mRNA and the protein was increased by circ_0000527 overexpression or miR-646 inhibition in RB cells, while knocking down circ_0000527 or upregulating miR-646 caused a decrease in LRP6 expression (Fig. 6c, d). The gene discussed is LRP6; the disease is retinoblastoma.