The results of ELISA showed that the levels of anti-TIF1γ-IgA and anti-TIF1γ-IgG in early LC group were significantly higher than that in LBL group and HC group (P<0.01, Figure 2A, B), while there was no significant difference in the expression of anti-TIF1γ-IgM and anti-TIF1γ-IgE among three groups (P>0.05, Figure 2C, D). The gene discussed is CD40LG; the disease is laryngotracheoesophageal cleft.