To determine whether ouabain can protect against vertical transmission of ZIKV, pregnant C57BL/6 mice were administered anti-Ifnar antibody at −1, +1, and +3 days relative to ZIKV infection, and then treated with ouabain at 3 mg/kg for 5 days (Fig. 5a). The gene discussed is IFNAR1; the disease is Zika virus infectious disease.