RAG1 and neoplasm: Consequently, the tumor sizes were less than 160 mm2 on day 28 post-B16F10-OVA injection, while those in the control (Rag1-/- transplanted with B16F10-OVA) group were around 400 mm2 on day 22 postinjection, leading to euthanasia to comply with experimental animal ethics procedures (figure 3B).