A study using in vitro co-cultures of PC-3 prostate cancer cells and fibroblasts demonstrated that the transmembrane EphrinB2 ligand interacts with its receptor EphB3/4 in PC-3 cells activating Cdc42 signaling, which stimulates cancer cell migration and causes a failure of contact inhibition of locomotion [135]. This evidence concerns the gene EFNB2 and cancer.