To explore the mechanism of up‐regulation of ASPM expression in gliomas, we utilized the JASPAR database and ENCODE database to find the potential transcription factors that may regulate the expression of ASPM, and finally, we obtained 18 transcription factors (EGR1, ETS1, GATA3, NFYA, NFYB, POU2F2, YY1, RUNX3, SP1, SP2, STAT1, BRCA1, CHD1, E2F6, FoxM1, FOXO4, REST and STAT3). The gene discussed is E2F6; the disease is central nervous system cancer.