In this study, we found for the first time that knockdown of ASPM can increase the expression levels of E‐cadherin protein in glioma cells and decrease the expression levels of N‐cadherin and Vimentin, MMP1, MMP2 and MMP9 proteins, suggesting that ASPM may affect the migration and invasion of glioma cells by regulating the expression of EMT‐related proteins. The gene discussed is CDH2; the disease is central nervous system cancer.