Cells underwent pressure treatment mimicking the glaucoma condition displayed a significant (p < 0.05) increase in optineurin (OPTN), beta-Arrestin-1, nestin, connexin 43, VCAM-1, and ICAM-1 proteins compared to control, while the cells had a pre-exposure to betalain exhibited the reduced levels of these deranged proteins suggest that the drug acts on the signaling and curtail them which are appealing towards malfunctioning. This evidence concerns the gene GJA1 and glaucoma.