NES and glaucoma: Cells underwent pressure treatment mimicking the glaucoma condition displayed a significant (p < 0.05) increase in optineurin (OPTN), beta-Arrestin-1, nestin, connexin 43, VCAM-1, and ICAM-1 proteins compared to control, while the cells had a pre-exposure to betalain exhibited the reduced levels of these deranged proteins suggest that the drug acts on the signaling and curtail them which are appealing towards malfunctioning.