We have previously described enhanced differentiation of primary rat osteoblasts and MC3T3‐E1 cells in 3D versus 2D culture, as well as increased sensitivity to inhibition of proliferation by a protein kinase inhibitor.(35) 3D culture alters the sensitivity of prostate cancer cells to antineoplastic drugs,(36) and enables spontaneous differentiation of mouse calvarial osteoblasts into osteocytes in the absence of any other factors.(37). The gene discussed is WEE1; the disease is prostate cancer.