BAIAP2 and colorectal adenocarcinoma: We confirm the apical localization of endogenous IRSp53, as well as of expressed GFP-IRSp53 in two-dimensional (2D) monolayers and 3D cysts of MDCK (Fig. 2a and Supplementary Fig. 2A) and colorectal adenocarcinoma (Caco-2) epithelial (Supplementary Fig. 2B–C) cells in vitro.