To evaluate whether GnRH agonist and r-hCG ovulation induction exerted in IVF surgery has an influence on the vaginal microbiome in infertile women, we compared alpha and beta diversity data between groups B-I and A-I, which revealed that GnRH agonist and r-hCG stimulation of ovulation exerted no influence on the vaginal microbiome in infertile women. This evidence concerns the gene GNRH1 and Infertility.