Target sequencing only detected genes exons related to cardiac development, we excluded the effects of other possible genes, and finally identified two mutant variants of FGF8 (Fig. 1b, d) in two patients with TOF (Additional file 1: Figure S1a, b) and one deleted variant of FGF10 (Fig. 1f) in a patient with single atrium, single ventricle, complete atrioventricular valve defect, and pulmonary valve stenosis (Additional file 1: Figure S1c). Here, FGF8 is linked to pulmonary valve stenosis.