Moreover, the frequency of circulating Bregs was positively correlated with serum levels of IL-10, IL-35 and BAFF among HCV-HCC group (r = 0.3, p = 0.01), (r = 0.27, p = 0.03) and (r =0.4, p = 0.001), respectively. The gene discussed is IL10; the disease is hepatocellular carcinoma.